The Louvre Museum has declined to comment on his claims because it "was not part of the scientific team".
Instead of the famous, direct gaze of the painting which hangs in the Louvre Museum in Paris, the image of the sitter also shows no trace of her enigmatic smile, which has intrigued art lovers for more than 500 years
But Mr Cotte's claims are controversial and have divided opinion among Leonardo experts.
The scientist, who is the co-founder of Lumiere Technology in Paris, was given access to the painting in 2004 by the Louvre. [What? It has taken 11 years to come out with this news???]
He has pioneered a technique called Layer Amplification Method (LAM), which he used to analyse the Mona Lisa. It works by "projecting a series of intense lights" on to the painting, Mr Cotte said. A camera then takes measurements of the lights' reflections and from those measurements, Mr Cotte said he is able to reconstruct what has happened between the layers of the paint.
'Shatter many myths'There has long been debate about the Mona Lisa's identity. But for centuries, it has been widely believed that she is Lisa Gherardini, the wife of a Florentine silk merchant.
The Secrets of the Mona Lisa is on BBC Two at 21:00 GMT on 9 December.